Common Mistakes When Using Variable Volume Pipettes
In the world of scientific research, accuracy is the difference between a breakthrough and a failed experiment. While variable volume pipettes are designed to provide flexibility and precision, human error can quickly compromise your results. Whether you are a seasoned researcher or a student using your first pipette starter kit, avoiding these common pitfalls will ensure your data remains reliable. 1. Dialing Outside the Volume Range One of the most frequent mistakes is trying to "push" a variable volume pipette beyond its stated limits. If a pipette is rated for 20–200 µL, cranking the dial to 205 µL won't give you a larger volume—it will likely damage the internal piston and throw the calibration off entirely. Always stay within the manufacturer’s specified range. 2. Ignoring the Pipette Angle For maximum accuracy, a pipette should be held vertically (at a 90° angle) during aspiration. Tilting the pipette even by 20 degrees can cause more liquid to enter the tip than ...